Jessie/Anime/Main series/History/BW

In the, Jessie, James, and Meowth were sent to the Unova region under orders from Giovanni after they were promoted in Memories are Made of Bliss!. Now wearing black uniforms instead of their previous white ones, Jessie and James, along with Meowth, followed various missions given to them by Giovanni through other people. To prevent them from getting too much attention, Giovanni did not allow their other Pokémon to be sent to them, only making them use Pokémon native to Unova. Unbeknownst to the two, Giovanni was not certain that they would be successful; however, he sees that their presence in the region will force Team Plasma to reveal themselves.

In Battling for the Love of Bug-Types!, Jessie and James were informed that there were no more missions for them currently. After that, they went back to wearing their white uniforms. The trio seems to be more serious and are much less comedic than before. They also appear much less often than in previous series.

Along with James and Meowth, Jessie reappeared in New Places... Familiar Faces!, returning to their original plan of catching Ash's Pikachu. They've also added new Pokémon to their team, with Jessie catching a sometime before meeting up with Ash and company. However, she is forced to give it and her away to Giovanni in The Dream Continues! under the pretense that she captured them for him. She also regained Wobbuffet as part of her team in the episode.