Jessie/Anime/Main series/History/OS

Jessie made her debut in the anime in Pokémon Emergency. Alongside James and Meowth, they set their sights on the injured Pokémon in the Pokémon Center. Sending out their and  from their Meowth balloon, they quickly overtook the Pokémon Center, which only had Nurse Joy,  and  inside. Before they could steal any of the Pokémon, however, Ash's Pikachu, who teamed up with many other in the Pokémon Center, used an electric attack, causing the entire Pokémon Center to explode. It was here that Team Rocket decided to capture Pikachu, deeming him as an exceptionally powerful Pokémon, and made it their goal to capture him.

In Electric Shock Showdown, after hearing that Pikachu would fight to defend his own honor and Ash's, the trio were inspired to cheer on Pikachu before his rematch against Gym Leader Lt. Surge's.

In The Punchy Pokémon, Jessie entered the P1 Grand Prix after stealing 's clothes and. She used Giant's clothes as a disguise. She did not make it as Hitmonlee lost to.

In Princess vs. Princess, Jessie participated in the Princess Festival. She caught a beforehand when it was caught stealing stuff from  with its tongue, claiming to deal with it later. Jessie competed in the contest of the Princess Festival but was eliminated by Misty despite her Lickitung nearly defeating the entirety of the team that Misty used.

In The Ultimate Test, Jessie decided to take the Pokémon League Admissions Exam to see if she could enter the Indigo Plateau Conference and add that achievement to her resumé. However, during the theory test, she raised from her seat and started to argue with the instructor, which led to her being expelled.

In Wobbu-Palooza!, she fought the Wobbuffet Festival crashers with her and single-handedly defeated them. When she, James, and Meowth were leaving the festival, they were caught with stolen food from the festival. Ash and his friends easily defeated them and got the food back.

In Control Freak!, she wore an ancient mask and scepter belonging to a queen from a long time ago. Using the items, Jessie possessed some Pokémon that she chose except for who was apparently unaffected. She was about to get away with the items but 's newly evolved Crobat sliced the Dig-a-tron and separated her from the mask and scepter.