Kanto Route 17/Anime

A Cycling Road first appeared in The Bridge Bike Gang, although is was notably different than in the games, connecting to Sunnytown instead of Fuchsia City. At first, and  were unable to cross it due their lack of bicycles, but to their luck, the local Nurse Joy allowed them to use loaned bicycles to deliver a medicine to the Sunnytown Pokémon Center. On the way, they were attacked by the local bike gang, led by Chopper. However, once the bike gang learned of the group's delivery mission, they let them pass and accompanied them all the way to Sunnytown.

A Cycling Road also appeared in The Green Guardian, this time connecting to Fuchsia City, like in the games. Ash and his friends intended to travel through it on their way to the, but found out that it was closed, forcing them to take a detour. It turned out that the road had been closed due to a group of huge vines having grown out of nowhere, blocking it off. With the help of the Solana, Ash and his friends found out that the cause of the vines was an injured, who had grown them in order to protect itself. After the group had helped Celebi recover, the Time Travel Pokémon made the vines disappear, opening the Cycling Road up once again and allowing Ash and his friends to use it to continue their journey.