Jessie/Anime/M20 & M21

Jessie appeared in I Choose You!, which is set in a different continuity from the main series. She first appeared in disguise with James and in a Pokémon Center, being wanted for stealing Pokémon. When a Trainer who had just battled an hurried inside the Pokémon Center, Team Rocket eavesdropped on the conversation and decided to hunt for Entei themselves. While looking for Entei in the forest, they were blasted off by a raging.

In "The Power of Us" Team Rocket hide behind some bushes as Ash meets Margo. Meowth asks how they are gong to steal Pikachu and Jessie suggests starting a juice business because that they will become rich. That day, before the catch race Team Rocket is in the background selling Lum Berries. Later that evening Team Rocket are trying to steal from Toren's lab Jessie reveals that she has "No idea" what moves and abilities are. Team Rocket is about to begin stealing when the Smeargle ink spills and Toren comes into the room to investigate so Team Rocket hides outside.

The next day while Toren is presenting his research, Harriet finds Team Rocket backstage and asks them who they are. Jessie starts saying the Team Rocket motto but Meowth interrupts them. James drops a smoke bomb and they run to the forest but while they are running Jessie thinks that she tossed the Effect Spore to Meowth but it turns out she didn't and she actually dropped it.

After the effect spore started spreading across the city, Team Rocket took their Lum berry cart and went to the Pokemon center. As they caught their breath Toren, with their permission, takes all of their Lum berries to stop the spread of and reverse the effects of effect spore.

